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Furman University

Greenville, South Carolina

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A START-UP MANUAL FOR UNDERGRADUATE RESEARCH STUDENTS IN MICROBIOLOGY
Active Learning from the Very Beginning
Keri Law, Rachel Lamb, and Min-Ken Liao

ADJUSTING pH
Dr. Lois Tvergerg

Fill the electrode with electrode buffer (in chemical cabinet) and close the stopper.

Calibration:

-If the pH meter hasn't been used in the last few days, calibrate it. You should calibrate using standards nearer to what you are measuring. We have pH 4, pH7, and pH 10 standards. If you are pH-ing something at 8.0, use the pH 7 and 10 standards to calibrate.

-Squirt about 5 ml of pH standard buffer into a 15 ml tube. (Don't use pH standard that has been used a long time ago and is still in the tube. The solution is not sterile, and the pH changes when bacteria grow in it.)

-Press Power to turn meter on. Put the electrode into standard. Then press 2nd, then cal. The number should finally hold on the right pH. (7.00 for the 7 standard, etc.) If the meter gives you E21 error message, you need to calibrate it manually. Read how to do this in the pH meter manual or ask me.