Prokaryote Translation: Initiation
Initiation
- Small ribosomal subunit (30S) binds S1 protein to activate the small subunit.
- Covers an area of 35-40 bp on the mRNA
- Activated small subunit binds to Shine-Dalgarno sequence 5’--AGGAGG--3’
- Lies 7 bases upstream from AUG start codon
- Complementary to 3’ end of 16S rRNA