Protein Denaturation

• Naive protein structure unfolds in a cooperative manner

  • Melting Temperature, Tm
  • pH:
  • Detergents
  • Water-soluble organic substances
  • Salts

• Hofmeister series:

  • Anions: SO42-?H2PO4->CH3COO-> CI- >Br- > I- >ClO4- >SCN-
  • Cations:NH4+>Cs+>K+ >Na+>Li+>Mg2+>Ca2+>BA2+
  • Ions in the Hofmeister series that tend to denature proteins
    • I- > ClO4- > SCN- , Li+>Mg2+>Ca2+>BA2+

Previous slide

Next slide

Back to first slide

View graphic version