Prokaryote: Termination

• UGA, UAG, UAA

  • no tRNAs for these 3 codons
  • Stop Codons

• Polypeptide is cleaved from last tRNA by releasing factors (RFs)

  • RF-1 recognizes UAA & UAG
  • RF-2 recognizes UAA & UGA
  • RF-3 binds GTP, enhances RF-1 & RF-2

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