1. how is DNA packaged with protein during mitosis? (What are the higher levels of structure?)
2. Why is a two step process more productive than a one step process of protein synthesis?
3. How does the polymerase "know" to read the sense strand? (several elements to this answer....)
4. What 'cues' determine where transcription will start and stop?
5. Consider the DNA double helix, below. Show what the RNA product will be after transcription. Remember - the RNA Polymerase lands on the promoter, and then reads one strand..... pick the correct one and transcribe it!
3'_________________________________5'....... __________
| | | | | | | | | | | | | | | |
A A T G C C C A T T G G C A | |
PROMOTER
T T A C G G G T A A C C G T | |
5' | | | | | | | | | | | | | | 3' ..... | |
6. What is an intitation complex?
7. Describe the translocation reaction.
8. How is a polypeptide modified after translation to become a functional protein?
9. What is the structure of a eukaryotic chromosome, and how is protein synthesis modified to accomodate this structural change?
10. What is the evolutionary implication of a universal code?