Proteins Titration Curves Are Complex

• Protein titration curves rarely provide indication of individual pK values

• Covalent & 3D structure of proteins cause pK of ionizable groups to shift several pH units from free ?-AA value

• Titration curves affected by salt concentration

  • Salt ions act electrostatically to shield side chain charges from one another
  • Charge-charge interactions become attenuated

Previous slide

Next slide

Back to first slide

View graphic version