Enzymes Can Be Assayed Quantitatively
Need following information for quantitation
1 unit of enzyme activity: amount causing transformation of 1 micromole (umol = 10-6mol) of substrate per min at 25oC under optimal conditions of measurement.
Specific activity: # of enzyme units per mg of protein
Turnover number: # of substrate molecules transformed per unit time by a single enzyme molecule when the enzyme conc. alone is the rate-limiting factor.
Substrate conc. has a profound effect on rate of enzyme- catalyzed reaction